Born at Lystra, Lycaenia, Timothy was the son of a Greek father and Eunice, a converted Jewish woman. He joined St. Paul when Paul preached at Lystra replacing Barnabas, and became Paul's close friend and confidant. Paul allowed him to be circumcised to placate the Jews, since he was the son of a Jewess, and he then accompanied Paul on his second missionary journey.
When Paul was forced to flee Berea because of the enmity of the Jews there, Timothy remained, but after a time was sent to Thessalonica to report on the condition of the Christians there and to encourage them under persecution, a report that led to Paul's first letter to the Thessalonians when he joined Timothy at Corinth. Timothy and Erastus were sent to Macedonia in 58, went to Corinth to remind the Corinthians of Paul's teaching, and then accompanied Paul into Macedonia and Achaia.
Timothy was probably with Paul when the Apostle was imprisoned at Caesarea and then Rome, and was himself imprisoned but then freed. According to tradition, he went to Ephesus, became its first bishop, and was stoned to death there when he opposed the pagan festival of Katagogian in honor of Diana. Paul wrote two letters to Timothy, one written about 65 from Macedonia and the second from Rome while he was in prison awaiting execution.